Between 2008 and 2011, with the new emergency treatment process in place, sufferers underwent catheter angiography at a median period of 2.0 hours after hospital admission . The correct treatment implemented at a median of 2.9 hours for endovascular coil placement or 3.1 hours for surgical aneurysm clip positioning after admission. The quickness with which catheter angiography and treatment commenced pursuing medical center admission did not differ significantly during daytime and nighttime hours. Eight patients developed in-medical center rebleeding during this period.DNA was extracted and used to sequence GNAQ and GNA11 and to perform array comparative genomic hybridization on a subgroup of samples. An in depth description of the techniques used for sequencing, cell tradition, comparative genomic hybridization, Western blotting, and in vivo tumorigenicity studies is offered in the Supplementary Appendix, obtainable with the full text of this article at NEJM.org. Results Sequencing We found mutations affecting Q209 in GNA11 in the same particular subgroups of melanocytic tumors that were previously described for GNAQ 11 .