New Optics for the development of the vaccine HIV

The seekers can have broken the impasse that has whipped the invention of an effective vaccine against the HIV bypassando the usual development method. With the transfer of technologies gene that produces molecules that to block the infection, the scientists have protected the monkeys from the infection of a virus strictly correlated to the virus HIV – the virus of the immunodeficiency of the monkeys (SIV) – that causes the Aids in the monkeys rhesus.

“We have used an equine strategy, bypassando the natural answer of the system immunitario that has been the target of all the proposed as a candidate precedents vaccinates HIV and SIV”, has said the head of the study Philip R. Johnson, close to the pediatric hospital of Philadelphia. Explaining his work on Natures Medicines, Johnson has informed that many obstacles remain before the technique used in this study on the animals might be translated in a vaccine against the HIV for the man.

The greatest part of the attempts started to develop a vaccine against the HIV have used substances turned to stimulate the system immunitario of the body of to produce antibodies and cells killer who would have eliminated the virus beforehand or later of him the infected cells in the body. The clinical studies still have been disappointing. He vaccinates against the HIV they have not provoked an answer immunitaria protective, really as the body does not manage to produce singly an effective answer against the HIV during the natural infection.

The approach adopted in this study has been divided into two phases. In the first phase, the research team has created antibodies as the proteins ​​ (immunoadhesins) that have been planned specifically to become attached to SIV and to block it to infect the cells. Once demonstrated of working against SIV in laboratory, DNA that represent specific SIV immunoadhesins has been planned in a virus vector planned to offer the monkeys DNA. The seekers have chosen virus adeno-associated (AAV) as the virus vector, because it is a very effective way to insert of the DNA in the cells of a monkey or human.

In the second part of the study, the seekers have injected vectors AAV in the muscles of the monkeys, where the DNA imported produced immunoadhesins what entered into the sanguineous circulation. One month after the somministrazione of the vectors AAV, the monkeys immunised they have been injected by the living one, which causes the Aids SIV. The greatest part of the immunised monkeys were completely proteges with the infection SIV, and they all were protected from Aids. To the contrary one, do not immunise a group of monkeys to you that they have been all infected by SIV, and two thirds Aids has died for complications. Upsetting, high concentrations of SIV-Specific immunoadhesins remained in the blood for more than one year.

“To have followed itself after all, the molecules more and better than they work against the HIV, included human monoclonal antibodies, it will be necessary,” noticed Johnson, adding that: “(the) approach can have also potential use in the prevention of other infectious sicknesses, like the malaria.”