Behind discovered mechanism of Salmonella virulence and drug sensitivity

The researchers also suggest that analysis of the way poxa yjeK and change the bacterial protein elongation factor is essential for the production of proteins that allow bacteria to use alternative energy sources when they are deprived of nutrients, such as E ‘the case after entering the host cells.The researchers found that these three genes function in a common pathway that is essential for the ability of Salmonella to cause disease and resist several classes of antibiotics. Salmonella with mutations in the gene or genes is poxa yjeK, study notes, seems to be almost identical and show similar changes in proteins involved in metabolism. Strains with mutations in two genes are similar to the single mutant strains, an observation that suggests that the two genes work the same way.

Salmonella enters the gut when people consume contaminated food, and can sometimes spread to other parts of the body. Salmonella outbreaks and recalls of groceries are often in the news. Infants, children, seniors and people with cancer or HIV are especially prone to serious illness of Salmonella.

Salmonella is adaptable and can withstand many of the body attempts to fight it. The bacteria live and multiply in a special compartment within the cells of a person or an infected animal. Salmonella can alter its physiology as it moves to a life free swimming at his residence in a host cell.

Future studies in his lab will focus on specific reasons behind the response to stress and bad bacteria yjeK poxa lacking and the explanation of its different effects on the quantities of individual proteins. The laboratory will also investigate the role of the normal protein and poxa yjeK as intriguing, in which the bacterial protein elongation is changed, the apparent universality of this mechanism for modifying proteins in living cells and its conservation in the course of ‘evolution.

Fang said: Sometimes it seems that the fundamental discoveries in biology have already been made. It ‘was fun and unexpected to learn something new about a fundamental process such as protein synthesis.

The broad spectrum of compounds that have significantly slowed the growth of these mutant strains suggest that the defect is in a general stress response, the researchers noted. The mutant bacteria measurably differed from the wild-type Salmonella in 300 different conditions. In addition, aberrant production of virulence factors reduces their ability to survive in the host.

Perhaps the mutant strains do not know when to stop. Wild-type Salmonella might enter a state of suspended animation to weather conditions, while the mutants fail to respond adequately to environmental stresses. The fact that the mutants continue to breathe when I’m in a terrible situation could lead to the production of toxic oxygen compounds.

The mutant strains exhibited many abnormalities under stress.

This is an interesting example of molecular evolution, said Fang. This fundamental mechanism not previously recognized to regulate protein production appears to have been preserved in the time evolution and continues to take place in cells belonging to all five kingdoms of life.

Unexpectedly, the researchers found that Salmonella with mutations in poxa yjeK and continued to breathe inappropriately in nutrient poor conditions in which the respiration of wild-type Salmonella cease.

The results were published on July 29 Molecular Cell Study author is Dr. C. Ferric Fang, professor of microbiology, medicine and laboratory medicine at the University of Washington . The author is William Wiley Navarre, who began the study as a postdoctoral fellow in the laboratory by Fang and is now an assistant professor at the University of Toronto.

This research was funded by the National Institute of Allergy and Infectious Diseases at the National Institutes of Health. Navarre has also received support from the Damon Runyon Cancer Foundation, Canadian Institutes of Health Research.

Stemedica Cell Technologies, Inc., a leader in the production of adult allogeneic research and development, announced today that its specially formulated ischemic adult allogeneic mesenchymal stem cells have been used successfully to treat the first patient in a study conducted at ischemic University of California, San Diego . A total of 35 patients will be enrolled in the clinical study entitled ‘An I / Phase II, multicenter, open-label study to evaluate the safety, tolerability and preliminary efficacy of a single intravenous dose of allogeneic bone marrow mesenchymal cells for subjects with ischemic stroke. ‘

This could explain, the authors suggested, because the mutants are broadly sensitive to a large number of unrelated compounds and cellular stress.

During the production of a protein, transfer RNA, also called tRNA, normally places an amino acid at the end of a chain of more and more bricks of proteins. A certain type of enzyme normally hands the tRNA the amino acid for this place.

However, in this study, the researchers showed for the first time that the enzymatic steps poxa and attaches directly to an amino acid of elongation factor P protein, rather than the tRNA.